SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)
Electrophoresis is based on the motion of charges in an electric field toward an electric charge. Macromolecules have differing mobilities based n thier charge, shape, and size.
SDS-PAGE uses polyacrylamide gel which offers higher resistance to large molecules than to small. This technique can be used to estimate molecular weights of proteins by comparing samples with the standard samples. |