AFFINITY CHROMATOGRAPHY
Affinity Chromatography uses the specific binding properties of many proteins. It is another form of column chromatography with a polymeric material used as the stationary phase. The distinguishing property of this technique is that the polymer binds specifically to the desired protein, while the other proteins in the sample do not bind to the column and can easily be eluted with buffer, while the bound proteins remains in the column. The bound protein then can be eluted on the column by adding high concentrations of the ligand in soluble form, thus competing for the binding of the protein with the stationary phase.
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